›› 2011, Vol. 23 ›› Issue (6): 416-420.doi: 10.3969/j.issn.1004-616x.2011.06.003

• 核辐射安全专家论坛 • Previous Articles     Next Articles

Differential gene expression profile in human hepatocytes irradiated by γ-rays after different time points

ZHANG Wei，QIN Xiu-jun，XU Chao-qi，LI Wei-bin，YIN Jing-jing，YUAN Hui，LI Jian-guo   

  1. Department of Radiation Medicine and Environment Medicine, China Institute for Radiation Protection, Taiyuan 030006, Shanxi, China
  • Received:2011-09-19 Revised:2011-09-22 Online:2011-11-30 Published:2011-11-30
  • Contact: ZHANG Wei

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Abstract: OBJECTIVE: To provide gene-level evidence for hepatic early damage of staff exposed to irradiation. METHODS：The study analyzed the differential gene experession profile of normal human hepatocytes and human hepatocytes irradiated at 2 different doses (0.5 and 1.0 Gy γ- rays) using whole genome chip after 4 and 24 h. RESULTS：There were 218 differentially expressed genes irradiated with 2 doses after 4 h，and there were 1 475 differentially expressed genes irradiated with 2 doses after 24 h. There were 235 differentially expressed genes irradiated with 0.5 Gy in 2 time-points. There were 170 differentially expressed genes irradiated with 1.0 Gy in 2 time points. There were 129 differentially expressed genes irradiated with 0.5 Gy and 1.0 Gy at 4 h and 24 h. Some interesting pathways such as insulin synthesis and secretion were found. The quantities of IGF2BP3 and EGR1 mRNA analyzed with real-time PCR were consisitent with gene chip. CONCLUSION：There were 129 differentially expressed genes identified. Irradiation caused early damage to human hepatocytes at many targets，many levels and through many pathways .

Key words: γ-rays, hepatocytes, gene chip, differential gene expression